Faculty & Research

Foreign Experts

Professor David P. Molloy

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PERSONAL INFORMATION:

Name: David P. Molloy

Gender: Male,

Nationality: UK

B.Sc., Ph.D., a highly motivated, efficient and confident senior scientist, with excellent communication, planning and organization skills with leadership and people management capabilities. Extensive knowledge and applications experience in protein biochemistry and molecular biology.

CONTACT INFORMATION

Tel : +86 134 6916 1937

e-mail: molloydp@yahoo.co.uk

RESEARCH INTERESTS

Structure-function of the chloroplast Mg-Proto IX methyltransferase CHLM protein and its role in and Chloroplast-nuclear retrograde signalling essential for stress-response and plant development.

Chloroplast editosomes and structural analysis of The Multiple Open Reading Frame (MORF) family proteins.

Structure-function of adenovirus early region 1A proteins (Ad E1A) in cellular transformation.

Analysis of Prion proteins involved in variant Creutzfeldt-Jakob disease (vCJD).

SKILLS

Heteronuclear, multi-dimensional NMR spectroscopy.

Protein structure calculations and analysis

Protein-protein dynamics, interactions and folding.

Fluorescence spectroscopy and rapid reaction kinetics.

UV/Vis spectroscopy and Circular Dichroism.

Bioinformatics and proteomics.

Computational modelling of protein structure

Western blotting, SDSPAGE, ELISAs and chromatography.

qRT-PCR, protein expression and purification.

EDUCATION

1991: Ph.D. (C.N.A.A.) Physical Biochemistry: National Institute for Medical Research, Mill Hill,

London NW7 1AA

1987: B.Sc. (C.N.A.A. HONS 2:1) Applied Biology; Portsmouth Polytechnic

1984: A-levels: Biology A; Environmental Studies, A; Chemistry, S; Farnborough College of Technology

1981: ‘O’-Levels: Religious Education, B. Biology, A. Geography, B. Chemistry, A Physics, A. Mathematics, A. French, B. English Language, A. English Literature, A. Economics, A: Salesian College Farnborough

PROFESSIONAL EXPERIENCE

Aug 2016 - present: Professor / Foreign Expert in Biochemistry and Molecular Genetics, Hunan University of Arts and Science, Changde, Hunan, P. R. China.

Jun 2011 - May 2016: Principal, Portsmouth International College, Portsmouth, Hampshire, England.

Jan 2004 - Jun 2005: Research Office Manager, Thames Valley University

Jul 2002 - May 2011: Senior Lecturer in Biosciences, Thames Valley University

2000 - 2002: Lecturer in Biochemistry, University of Luton

1992 - 2000: Research Fellow / Senior Research Fellow, University of Birmingham

1990 - 1992: Research Fellow, Department of Biochemistry, Baylor College of Medicine, Houston, TX, U.S.A.

OTHER ACTIVITIES

Member of London Structural Biology Club

Member of The American Chemical Society

PUBLICATIONS

Huang, C., Li, Ye, L-S., Yu, Q-B., Cui, Y-L., Molloy, D.P. and Yang, Z-N. (2019) Mutation of chloroplast CHLM contributes to down-regulation of CSD2, FSD1 and CBF3 nuclear genes in Arabidopsis. Anal.Botany. submitted

Huang, C., Li, Z-R., Yu, Q-B., Cui, Y-L., Molloy, D.P. and Yang, Z-N. (2019) MORF2 tightly associates with MORF9 to regulate chloroplast RNA editing in Arabidopsis. Plant Sci. 278: 64-69

Grand, R.J.A and Molloy, D.P. (2009) The relationship of structure to function of the adenovirus early region 1 A oncoprotein, chapter 8 pp1-36 in Molecular Biology of Tumour Virus Gene Products (Ed Yoshida, K) ISBN 978-81-308-0324-1

Molloy, D.P., Barral, P.M., Gallimore, P.H. and Grand, R.J.A. (2007) The effect of CtBP1 binding on the structure of the C-terminal region of adenovirus 12 early region 1A. Virology 363: 342-356.

Chen, B. and Molloy, D.P. (2007) Potential sites on Prion and Dopple polypeptides responsible for interactions with therapeutic agents for the treatment of TSEs. Journal of Molecular Modelling 13: 891-896

Molloy, D.P., Webster, R., Gallimore, P.H. and Grand, R.J.A. (2006) Acetylation at a lysine residue adjacent to the CtBP binding motif within adenovirus 12 E1A causes structural disruption and inhibition of binding. Virology 355:115-126.

Molloy, D.P. and Chen, B. (2005) Predicted consequences of site-directed mutagenesis and the impact of species variation on prion protein misfolding through the N-terminal domain. J. Molecular Modelling 11: 468-473

Molloy, D.P., Barral, P.M., Bremner, K.H, Gallimore, P.H. and Grand, R.J.A. (2001) Structural determinants outside the PXDLS sequence affect the interaction of adenovirus E1A, C-terminal interacting protein and Drosophila repressors with C-terminal interacting protein. Biochimica et Biophysica Acta. 1546: 55-70

Molloy, D.P., Barral, P.M., Bremner, K.H, Gallimore, P.H. and Grand, R.J.A. (2000) Structural determinants in Adenovirus 12 E1A involved in the interaction with C-terminal Binding Protein 1. Virology 277:156-166

Molloy, D.P., Smith, K.J., Milner, A.E., Gallimore, P.H. and Grand, R.J.A (1999) Structure of the region on adenovirus E1A responsible for binding to TATA binding protein. J. Biol. Chem. 274: 3503-3513.

Molloy, D.P., Milner, A.E., Yacub, I., Smith, K.J., Chinnadurai, G., Gallimore, P.H. and Grand, R.J.A. (1998) Structural determinants present in the CtBP binding site of adonvirus E1A. J. Biol. Chem. 273: 20867-20876.

Grand, R.J.A., Gash, L., Milner, A.E., Molloy, D.P., Turnell, A. and Gallimore, P.H. (1998) Regeneration of the binding properties of adenovirus 12 early region 1A proteins after preparation under denaturing conditions. Virology 244: 230-242.

Mills, C.O., Milkiewicz, P., Molloy, D.P., Baxter, J., and Elias, E. (1997) Synthesis, physical and biological properties of Lithocholyl-lysyl-fluorescein: A fluorescent mono-hydroxy bile salt analogue with cholestatic properties. Biochem. Biophys. Acta. 1336: 485-496.

Zera, E.M., Molloy, D.P., Angelson, J.K., Lamture, J.A., Wensel, T.G. and Malinski, J.A. (1996) Low affinity interactions of GDPS and ribose- or phosphoryl-substituted GTP analogues with the heterotrimeric G-protein, Transducin. J. Biol. Chem. 271: 12925-12931.

Molloy, D.P., Owen, D. and Grand, R.J.A. (1995) Ras binding to a C-terminal region of GAP. FEBS lett. 368: 297-303

Zera, E.M., Molloy, D.P., Wensel, T.G., Lamture, J.A., Angelson, J.K. and Malinski, J.A. (1993) Interaction of photoreceptor G protein, Transducin, with guanine nucleotide analogues. Biophys. J. 64: A383.

Keane, N.E. Molloy, D.P., Grand, R.J.A., Owen, D., Levine, B.A. and Ellis, L. (1993) Peptide mimetics of nucleotide phosphate binding sites. Biochem. Soc. Trans. 21: 267s.

Eccleston, J.F. Molloy, D.P., Hinds, M.G., King, R.W. and Feeney, J.J. (1993) Conformational differences between complexes of elongation factor Tu studied by 19F NMR. Eur.J. Biochem. 93: 1041-1047

Molloy, D.P. and Eccleston, J.F. (1990) Interaction of fluorescent analogues of guanine nucleotides with elongation factor Tu from E. coli. J. Cell. Biochem. 14C:162.

Eccleston, J.F., Kanagasabai, T.K., Molloy, D.P., Neal, S.E. and Webb, M.R. (1989) The application of fluorescent and photosensitive analogues of guanine nucleotides to the function and structure of G-binding proteins pp87-97 in: The guanine nucleotide binding proteins (Bosch et al eds) NATO series ii. Plenum Publishing Corporation. ISBN 978-1-4757-2037-2

REFEREES

Dr R.J.A. Grand, Reader.

Cancer UK Institute for Cancer Studies, University of Birmingham, Vincent Drive,

Edgbaston, Birmingham B15 2TA.

e-mail: r.j.a.grand@bham.ac.uk

Tel: 0121 414 2805

Professor B. Chen.

Deparment of Chemistry, University of Sheffield, Dainton Building, Brook Hill, Shefield, S3 7NU.

e-mail: b.chen@shefield.ac.uk

Tel: 0114 22 29467