PERSONAL INFORMATION
Name: Shipeng Shao
Gender: Male
Title: Prof.
CONTACT INFORMATION
Email: ssp@cqmu.edu.cn
Address: 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, P.R.China
Phone: +86-18510211543
EDUCATION EXPERIENCE
2013-2018 Ph.D. in Biophysics, School of Life Science, Peking University, Beijing, China. Thesis: Single Molecule Study of Chromatin-associated Dynamics in Living Cells. Advisor: Yujie Sun.
2009-2013 B.A. in Bioengineering, School of Chemistry Engineering, Sichuan University, Chengdu, China.
WORKING EXPERIENCE
2020-2022 Professor. School of Basic Medical Sciences，ChongQing Medical University.
2020-2022 Assistant investigator. Beijing Anzhen Hospital.
2018-2020 Postdoc. in Biophysics, School of Life Science, Peking University, Beijing, China. Advisor: Cheng Li.
RESEARCH INTERESTS
1. Interphase Chromatin Dynamics of Mammalian Cells
Development and application of sequence-specific chromatin labeling and imaging techniques in living cells (modified sgRNA of the CRISPR/Cas9 system and sgRNA multiplexing expressing system) to study the dynamic changes of chromatin position and structure in mammalian cells. Using genome editing technique to screen nuclear scaffold protein coding genes that have influence on the dynamics of chromatin in interphase. Development and application of living cell single molecule imaging and tracking technique to study the interaction between dimeric transcriptional factors (c-Fos and c-Jun, YAP and TAZ) and chromatin, thus dissecting the role of dimeric transcriptional factors in organizing chromatin structure.
2. Phase Separation in Organizing Chromatin Structure and Transcription Factories
Using live cell fluorescence imaging, super resolution imaging, single molecule tracking, and in vitro reconstitution methods to study phase separation mechanism in controlling mammalian genome structure and function, regulation of chromatin structure and transcription. Probing the role of nuclear actin and N-WASP in regulation the RNA Pol II transcription with a new perspective of phase separation.
HONOUR & AWARDS
2018 |
Beijing Outstanding Graduates |
2017 |
Peking University Excellent Study Pacemaker |
2017 |
Peking University Graduate Innovation Award |
2017 |
Graduate National Scholarship |
2017 |
Peking University President Scholarship |
2016 |
Peking University Graduate Innovation Award |
2016 |
Graduate National Scholarship |
2016 |
Peking University President Scholarship |
2013 |
Sichuan Province Outstanding Graduates |
2013 |
Excellent Graduate Cadres |
2012 |
First prize of "East Fulong" Cup second national pharmaceutical engineering design competition |
2012 |
Outstanding Student Leaders |
2012 |
Outstanding Student |
2012 |
Comprehensive Scholarship |
2012 |
National Scholarship |
2011 |
Outstanding Student Leaders |
2011 |
Comprehensive Scholarship |
2011 |
National Scholarship |
2010 |
National Inspirational Scholarship |
2007 |
First Prize of Jiangsu Provincial Biological Contest |
PUBLICATIONS
1. Hongchen Zhang#, Shipeng Shao#*, Yong Zeng, Xiaotian Wang, Yizhi Qin, Qiunan Ren, Shengqi Xiang, Yuxin Wang, Junyu Xiao, Yujie Sun*. (2022). Reversible phase separation of HSF1 is required for an acute transcriptional response during heat shock. Nature Cell Biology, 2022, 24, 340–352.
2. Shipeng Shao, Weiwei Zhang, Huan Hu, Boxin Xue, Jinshan Qin, Chaoying Sun, Yuao Sun, Wensheng Wei, and Yujie Sun, Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system. Nucleic acids research, 2016. 44(9): p. e86-e86.
3. Lei Chang#, Mengfan Li#, Shipeng Shao#, Boxin Xue, Yingping Hou, Ruifeng Li, Cheng Li, and Yujie Sun, Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells. Protein & cell, 2020. p. 1-23.
4. Shipeng Shao, Lei Chang, Yuao Sun, Yingping Hou, Xiaoying Fan, and Yujie Sun, Multiplexed sgRNA expression allows versatile single nonrepetitive DNA labeling and endogenous gene regulation. ACS synthetic biology, 2017. 7(1): p. 176-186.
5. Shipeng Shao, Hongchen Zhang, Yong Zeng, Yongliang Li, Chaoying Sun, Yujie Sun, TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells. Communications biology, 2021. 4(1): p. 1-14.
6. Shipeng Shao, Boxin Xue, and Yujie Sun, Intranucleus single-molecule imaging in living cells. Biophysical journal, 2018. 115 (2), p. 181-189.
7. Shipeng Shao, Lei Chang, Yingping Hou, and Yujie Sun, Illuminating the structure and dynamics of chromatin by fluorescence labeling. Frontiers in Biology, 2017. 12(4): p. 241-257.
8. Hongchen Zhang, Shipeng Shao*, Yujie Sun*. (2022). Characterization of liquid-liquid phase separation using super-resolution and single-molecule imaging. Biophysics Reports, 2022, 7, 1-12.
9. Yuan Wang, Chunlei Zhang, Wenzhong Yang, Shipeng Shao, Xinmin Xu, Yujie Sun, Pilong Li, Ling Liang, Congying Wu, LIMD1 phase separation contributes to cellular mechanics and durotaxis by regulating focal adhesion dynamics in response to force. Developmental Cell, 56 (9), 1313-1325. e7.
10. Mian Wei, Xiaoying Fan, Miao Ding, Ruifeng Li, Shipeng Shao, Yingping Hou, Shaoshuai Meng, Fuchou Tang, Cheng Li, Yujie Sun, Nuclear actin regulates inducible transcription by enhancing RNA polymerase II clustering. Science Advances, 6 (16), eaay6515.
11. Yongliang Li, Shipeng Shao, Xiao Xu, Xiaodong Su, Yujie Sun, and Shicheng Wei, MapZ Forms a Stable Ring Structure That Acts as a Nanotrack for FtsZ Treadmilling in Streptococcus Mutans. ACS nano, 2018.
12. Lei Chang, Gang Sheng, Yiwen Zhang, Shipeng Shao, Yanli Wang, Yujie Sun, AgoFISH: cost-effective in situ labelling of genomic loci based on DNA-guided dTtAgo protein. Nanoscale Horizons, 4 (4), 918-923.
13. Xue Boxin, Zhou Caiwei, Qin Yizhi, Li Yongzheng, Sun Yuao, Chang Lei, Shao Shipeng, Li Yongliang, Zhang Mengling, Sun Chaoying, He Renxi, Peter Su Qian, Sun Yujie, PN-ImTLSM facilitates high-throughput low background single-molecule localization microscopy deep in the cell. Biophysics Reports, 7 (4), 313-325.
