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College of Laboratory Medicine -- Associate Professor LI Junjie

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PERSONAL INFORMATION

Name: Junjie Li

Gender: Male

Title: Doctor (Dr.)

Position: Associate Professor/ Associate Researcher

 

CONTACT INFORMATION

Email: jjayli2006@cqmu.edu.cn, lijunjie2006@gmail.com 

Address: Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, P. R. China

Phone: +86-15823360689

 

EDUCATION EXPERIENCE

2018 - 2021, postdoc., Chongqing Medical University, P.R. China

2015 - 2017, Visiting scholar, University of Illinois at Urbana-Champaign, USA

2006 - 2015, Bachelor, Master and phD, Chongqing University, P.R. China

 

WORKING EXPERIENCE

2021 - now, Associate Professor, Chongqing Medical University, P. R. China

2018 - 2021, postdoc, Chongqing Medical University, P. R. China

 

RESEARCH INTERESTS

Molecular Diagnosis: In pursuit of advanced and globally applicable molecular diagnostic methodologies, we seek to amalgamate emerging biosensing techniques with established clinical diagnostic practices. Our overarching objective is to devise accessible clinical tools and analytical methods for biomarker assessment. This endeavor leverages state-of-the-art technologies such as CRISPR-Cas Technology, Isothermal Nucleic Acid Amplification Techniques, Multiplex PCR, and Microfluidic Chips. We place particular emphasis on the deployment of these methodologies for the real-time detection of bloodstream infectious pathogens, molecular subtyping of tumors, and companion diagnostics for anticancer therapeutics.

Functional Nano/Bio-materials: Employing nanomaterials as carriers and amalgamating them with functional nucleic acids or innate biocatalysts, our objective is to engineer monodisperse materials with exceptional stability, specificity, and reproducibility. These biofunctionalized nanomaterials are envisioned for applications encompassing in situ cellular imaging, precise targeted drug delivery, as well as the separation and enrichment of biomacromolecules.

Protein Engineering: Through the integration of computer-aided design, molecular cloning methodologies, and advanced protein engineering techniques, we undertake the refinement and customization of natural enzymes, with the primary objective of enhancing their catalytic efficacy and structural robustness. These meticulously engineered enzymes assume pivotal roles in diverse applications, including biocatalysis, gene editing, and isothermal nucleic acid amplification.

  

PUBLICATIONS 

  1. Wu You, Luo Wang, Li Junjie*, Yang Yujun*, Xie Guoming*, A PAM-free CRISPR-Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration. Nucleic Acid Research 2022, 50(20), 11727-11737.

  2. Bai Dan, Zhou Xi, Luo Wang, Yu Hongyan, Bai Shulian, Wu You, Song Lin, Chen Kena, Xie Yaxing, Chen Xueping, Zhao Jie, Fu Yixin, Yang Yujun*, Li Junjie*, Xie Guoming*. Cooperative strand displacement circuit with dual-toehold and bulge-loop structure for single-nucleotide variations discrimination. Biosensors and Bioelectronics 2022, 216, 114677.

  3. Lin Song#, Junjie Li#*, Kena Chen, Chen Zuo, You Wu, Dan Bai, Lin Zhao, Yujun Yang, Chenggui Liu*, Guoming Xie*. Site-specific insertion of endonuclease recognition sites into amplicons to improve post-PCR analysis sensitivity of gene mutation. Biosensors and Bioelectronics 2022, 208, 114191.

  4. Weng Zhi, Hongyan Yu, Wang Luo, Yongcan Guo, Qian Liu, Li Zhang, Zhang Zhang, Ting Wang, Ling Dai, Xi Zhou, Xiaole Han, Luojia Wang, Junjie Li*, Yujun Yang*, and Guoming Xie*. Cooperative Branch Migration: A Mechanism for Flexible Control of DNA Strand Displacement. ACS Nano 2022, 16, 3135-3144.

  5. Wang Yufeng, Guo Yongcan, Zhang Li, Yang Yujun, Yang Shuangshuang, Yang Liu, Li Junjie*, Xie Guoming*. Integration of multiplex PCR and CRISPR-Cas allows highly specific detection of multidrug-resistant Acinetobacter Baumannii. Sensors and Actuators B: Chemical 2021, 334, 129600.

  6. Li Junjie#*, Yang Shuangshuang, Zuo Chen, Dai Ling, Guo Yongcan*, Xie Guoming*. Applying CRISPR-Cas12a as a Signal Amplifier to Construct Biosensors for Non-DNA Targets in Ultralow Concentrations. ACS sensors 2020, 5(4):970-7.

  7. Bai Shulian, Xu Bangtian, Zhang Yangli, Zhang Yuhong, Dang Hao, Yang Shuangshuang, Zuo Chen, Zhang Li, Li Junjie*, Xie Guoming*. Tuning the specificity of DNA probes using bulge-loops for low-abundance SNV detection. Biosensors and Bioelectronics 2020, 154: 112092.

  8. Yang Shuangshuang, Guo Yongcan, Fan Jia, Yang Yujun, Zuo Chen, Bai Shulian, Sheng Shangchun, Li Junjie*, Xie Guoming*. A fluorometric assay for rapid enrichment and determination of bacteria by using zirconium-metal organic frameworks as both capture surface and signal amplification tag. Microchimica Acta 2020, 187(3):1-0.

  9. Yu Shengsong, Li Junjie#*, Cui Chang, Tian Shiliang, Chen Jiejie, Yu Hanqing, Hou Changjun, Nilges MJ*, Lu Yi*. Structural Basis for a Quadratic Relationship between Electronic Absorption and Electronic Paramagnetic Resonance Parameters of Type 1 Copper Proteins. Inorganic Chemistry 2020, 20, 59(15):10620-7.

  10. Li Junjie#, Hou Changjun*, Huo Danqun, et al. Detection of trace nickel ions with a colorimetric sensor based on indicator displacement mechanism. Sensors and Actuators B: Chemical 2017, 241, 1294-1302.

  11. Li Junjie#, Fu Beibei#, Hou Changjun*, Huo Danqun, et al. Discrimination of Chinese teas according to major amino acid composition by a colorimetric IDA sensor. Sensors and Actuators B: Chemical 2017, 240, 770-778.

  12. Li Junjie#, Wang Xianfeng#, Hou Changjun*, Huo Danqun, et al. Colorimetric measurement of Fe3+ using a functional paper-based sensor based on catalytic oxidation of gold nanoparticles. Sensors and Actuators B: Chemical 2017, 242, 1265-1271.

  13. Ji Chenhui#, Li Junjie#, Hou Changjun*, Huo Danqun, et al. Mesoporous hollow silica shells modified with functional diamine groups show high-performance absorption capacity and selective colorimetric response to copper ions in aqueous solutions. Sensors and Actuators B: Chemical 2017, 240, 718-725.

  14. Li Junjie#, Ji Chenhui#, Hou Changjun*, Huo Danqun, et al. High efficient adsorption and colorimetric detection of trace copper ions with a functional filter paper. Sensors and Actuators B: Chemical 2016, 223, 853-860.

  15. Li Junjie#, Hou Changjun*, Huo Danqun, et al. Development of a colorimetric sensor array for the discrimination of aldehydes. Sensors and Actuators B: Chemical 2016, 196, 10-17.

  16. Li Junjie#, Song Chunxia, Hou Changjun*, Huo Danqun, et al. Development of a colorimetric sensor array for the discrimination of Chinese liquors based on selected volatile markers determined by GC-MS. Journal of agricultural and food chemistry 2014, 62(43), 10422-10430.

  17. Li Junjie#, Hou Changjun*, Huo Danqun, et al. Molecularly imprinted polymers on the surface of silica microspheres via sol‐gel method for the selective extraction of streptomycin in aqueous samples. Journal of separation science 2013, 36(6), 1142-1148.

  18. Li Junjie#, Hou Changjun*, Huo Danqun, et al. A colorimetric multilayer sensor for discriminating red wine and green tea by measurement of antioxidant activity. Analytical Methods 2016, 8(16), 3345-3352.


PERSONAL WEBSITE

https://scholar.google.com/citations?user=4bC-kN8AAAAJ&hl=en

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