PERSONAL INFORMATION
Name: Xiaolan Yang
Gender: Female
Title: PhD, Prof. of Medicinal Chemistry
CONTACT INFORMATION
Email: xiaolanyang666@cqmu.edu.cn
Address: Key Laboratory of Medical Laboratory Diagnostics of the Education Ministry, College of Laboratory Medicine, Chongqing Medical University, No.1, Yi Xue Yuan Road, Daping, Yuzhong District, Chongqing 400016, China.
Phone: +86--23-68485240
EDUCATION EXPERIENCE
1987/09~1991/07, Bachelor of Science, Chemistry, Southwest Normal University, Chongqing, China
1991/09~1994/07, Master of Science, Organic Chemistry, Beijing Normal University, Beijing, China
2003/09~2006/07, Doctor of Science, Pharmacology, Chongqing Medical University, Chongqing, China
2021/12 to 2022/03, Visiting Scholarship, Department of Chemistry, Hong Kong University of Science and Technology
WORKING EXPERIENCE
1994/07~2006/07, Department of Chemistry, Chongqing Medical University, China
2006/08~2010/12, College of Pharmaceutical Sciences, Chongqing Medical University, China (2006/09, Associate Professor of Pharmaceutical Chemistry; 2011/09, Professor of Pharmaceutical Chemistry)
2011/01~present, Master's Supervisor, Doctoral Supervisor, College of Laboratory Medicine, Chongqing Medical University, China
RESEARCH INTERESTS
1. New methods and materials for sensitive and specific analysis of marker;
2. Design, synthesis and screening of enzyme inhibitors;
3. Magnetic separation facilitated rapid discovery of high-affinity ligands in trace contents from Traditional Chinese Medicines.
HONOUR & AWARDS
2023/08-2026/07, Chongqing Science and Technology Commission, No. CSTB2023NSCQ- MSX0994.
2023/01-2026/12, National Natural Science Foundation of China, No. 82273900.
2019/07-2022/06, Chongqing Science and Technology Commission, No. CSTC2019jcyj-msxmX0166.
2016/01-2019/12, National Natural Science Foundation of China, No. 31570862; 2012/09-2015/09, Chongqing Science and Technology Commission, No. CSTC 2012JJA0057.
2011/09~2012/12, Ministry of Science and Technology, “863”, No. 2011AA02A108, Sub-Project
2010/06~2012/12, Chongqing education committee, No.KJ100313.
PUBLICATIONS
1. Qiaomin Wu, Xinni Xiang, Yixiao Yuan, Yang Yu, Mengqi Chen, Jinyan Long, Tingxiu Xiang*, Xiaolan Yang*. GlaI cleavage assistant isothermal exponential amplification coupling with CRISPR/Cas12a for ultrasensitive detection of CLDN11 methylation: A potential marker for lung adenocarcinoma, Sensors and Actuators B: Chemical, 2023, 385: 133675, ISSN 0925-4005, https://doi.org/10.1016/j.snb.2023.133675
2. Jinyan Long, Mengqi Chen, Yang Yu, Qiaomin Wu, Xiaolan Yang*. Triple-recognition strategy for one-pot detection of single nucleotide variants by aligner-mediated cleavage-triggered exponential amplification, Analytica Chimica Acta, 2023, 1276: 341617, ISSN 0003-2670, https://doi.org/10.1016/j.aca.2023.341617.
3. Yang Yu, Hongwei Zeng, Qiaomin Wu, Xue Jiang, Changyuan Duan, Jinyan Long, Mengqi Chen, XiaolanYang*. A sensing strategy combining T7 promoter-contained DNA probe with CRISPR/Cas13a for detection of bacteria and human methyltransferase, Analytica Chimica Acta, 2022, 1227:340266, DOI: 10.1016/j.aca.2022.340266
4. Hongwei Zeng, Penghui Zhang, Xue Jiang , Changyuan Duan , Yang Yu, Qiaoming Wu, Xiaolan Yang*. Rapid RNA detection through intra-enzyme chain replacement-promoted Cas13a cascade cyclic reaction without amplification, Analytica Chimica Acta 2022,1217:340009, DOI: 10.1016/j.aca.2022.340009
5. Changyuan Duan, Qihua Jiang, Xue Jiang, Hongwei Zeng, Qiaomin Wu, Yang Yu, Xiaolan Yang,* Discovery of a Novel Inhibitor Structure of Mycobacterium tuberculosis Isocitrate Lyase. Molecules 2022, 27, 2447. https://doi.org/10.3390/molecules 27082447
6. Bangtian Xu#, Tingting Tong#, Xin Wang#, Fang Liu#, Xiang Zhang, Xiaolei Hu, Xinpeng Li, Xiaolan Yang*, Fei Liao*. Short divalent ethacrynic amides as pro-inhibitors of glutathione S-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers. Journal of Enzyme inhibition and Medicinal Chemistry. 2022, 37(1), 728-742 DOI:10.1080/14756366.2022.2038591
7. Xue Jiang, Hongwei Zeng, Changyuan Duan, Qianfang Hu, Qiaomin Wu, Yang Yu, Xiaolan Yang*. One-pot synthesis of stable and functional hydrophilic CsPbBr3 perovskite quantum dots for “turn-on” fluorescence detection of Mycobacterium tuberculosis. Dalton Transactions. 2022, 51, 3581. DOI:10.1039/d1dt03624f.
8. Xinpeng Li, Qihua Jiang*, Xiaolan Yang*. Discovery of inhibitors for Mycobacterium tuberculosis peptide deformylase based on virtual screening in silico. Molecular Informatics. 2021, 40, 2100002. DOI: 10.1002/minf.202100002 (IF 4.03)
9. Xiang Zhang, Shu He, Xiaolei Hu, Jing Wu, Xinpeng Li , Fei Liao*, Xiaolan Yang*. Comparison of the Full-Length and 152~528 Truncate of Human Cyclic Nucleotide Phosphodiesterase 4B2 for the Characterization of Inhibitors. Comb Chem High Throughput Screen. 2019;22 (1):49-58.
10. Yaping Li, Huimin Chong, Xiang Zhang, Xiaolan Yang*, Fei Liao*. High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag, Anal Biochem, 2020; 588:113474
11. Tong Chen, Linyu Yang, Xiaolan Yang*, Fei Liao*. Ion-exchangemediumcoatedwithabundantsmallzwitterionsforthepurificationofsolubleproteins. Prep Biochem Biotech, 23 Sep 2020 online, doi:10.1080/10826068.2020.1821218
12. Chao Xia#, Jinpeng Lu#, Bangtian Xu, Xiaolei Hu, Yixian Jing, Linyu Yang, Xinpeng Li,Wei Zhou, Gaobo Long, Fei Liao*, Xiaolan Yang*. Design and Characterization of a Labeling Reagent for Covalent Immobilization of Glutathione-S-Transferase. Nanosci Nanotechn Lett, 2019, 11(11):1547-1560.
13. Mingyue Zou, Xiaolei Hu, Bangtian Xu, Tingting Tong, Yixian Jing, Lei Xi,Wei Zhou, Jingpeng Lu, Xin Wang, Xiaolan Yang*, Fei Liao*(co-corresponding author). Glutathione Stransferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer, Oncology Reports 2018,41(2): 989-998
14. Jingjing Rao, Juan Liaob, Youquan Bu, Yitao Wang, Xiaolei Hu, Gaobo Long, MingtongHuang, Luhui Zhong, Xiaolan Yang*, Fei Liao*. Ampholytic ion-exchange materials coated with small zwitterions for high-efficacy purification of ionizable soluble biomacromolecules., Int J Biol Macromol 2018,120:2234-2241
15. Yang Xiaolan, Feng Yiran, Chong Huimin, Wang Deqiang, Hu Xiaolei, Pu Ju, Zhan Chang-guo, Liao Fei*. High-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins. Anal Biochem 2017;534:91-98
16. Jun Pu, Xiaolei Hu, Juan Liao, Yuwei Li, Jialin Qin, Yanling Xie, Chang-Guo Zhan, Xiaolan Yang*, Fei Liao*. Achievement of linear response for competitive bioaffinity assay of ligand: criteria of optimized interaction system. RSC Adv 2016, 6(112): 110858-110865
17. Chun Zhang#, Jun Pu#, Xiaolan Yang#(co-first), Tao Feng, Fang Liu, Deqiang Wang, Xiaolei Hu, Ang Gao, Hongbo Liu, Chang-Guo Zhan*, Fei Liao*. Extracorporeal delivery of a therapeutic enzyme. Sci Report 2016; 6:30888
18. Xiaolan Yang, Xiaolei Hu, Chunyan Chen, Gaobo Long, Jing Wu, Deng Tan, Yuanli Li, Yiran Feng, Jun Pu, Fei Liao*. Comparison of the immobilization of 6His-tagged proteins on magnetic-submicron-particle functionalized with Ni2+-NTA and bis-sulfone. Nanosci Nanotechn Lett 2015, 7(6):486-494.
19. Xiaolan Yang, Xiaolei Hu, Bangtian Xu, Xin Wang, Jialin Qin, Chenxiong He, Yanling Xie, Yuanli Li, Lin Liu, Fei Liao*. Fluorometric titration approach for calibration of quantity of binding site of purified monoclonal antibody recognizing epitope/hapten nonfluorescent at 340 nm. Anal Chem 2014, 86(12):5667-5672
20. Xiaolan Yang, Zhiyin Du, Jun Pu, Hua Zhao, Hong Chen, Yin Liu, Zhirong Li, Zhenli Cheng, Huansi Zhong, Eei Liao*.Classification of difference between inhibition constants of an inhibitor to facilitate identifying the inhibition type. J Enzyme Inhibit Med Chem 2013,28(1):205-213.
21. Hongbo Liu#, Xiaolan Yang#(co-first), Lin Liu, Jizheng Dang, Yanling Xie, Yi Zhang, Jun Pu, Gaobo Long, Yuanli Li, Yonghua Yuan, Juan Liao, Fei Liao*. Spectrophotometric-dual-enzyme-simultaneous-assay in one reaction solution (SDESA): chemometrics and experimental models. Anal Chem 2013, 85 (4):2143-2154.
22. Xiaolan Yang, Yonghua Yuan, Chang-Guo Zhan*, Fei Liao*. Uricases as therapeutic agents to treat refractory gout: current states and future directions. Drug Dev Res.2012, 73(2):66-72
23. Xiaolan Yang, Jun Pu, Hua Zhao, Xiaoyan Li, Juan Liao, Yanling Xie, Sha Zhu, Gaobo Long, Yonghua Yuan, Fei Liao.Method to screen aromatic ligands in mixtures for quantitative affinities to target using magnetic separation of bound ligands along with HPLC and UV photometry detection. Michrochim Acta 2012, 176(1):243-249.
24. Xiaolan Yang, Yanling Xie , Jun Pu, Hua Zhao, Juan Liao, Yiwen Chen, Gaobo Long, Chun Zhang, Yonghua Yuan, Sha Zhu, Huidong Yuan, Fei Liao*. Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model.BMC Biotechnol 2011, 11:44
Patents
1. Xiaolan Yang,Xue Jiang,Xiaolei Hu. Preparation of a functionalized hydrophilic lead halide perovskite quantum dot and its biosensing application.
ZL202110536658.4, patented March 20,2023
2. Xiao-lan Yang, Shu He, Fei Liao, Xiaolei Hu, Xiang Zhang, Chun-yan Chen.Benzamide derivatives with saturated small ring amine as PDE4B inhibitors. Chinese patent, ZL 201610093456.6 CN105646399B, patented Mar.8, 2019.
3. Xiao-lan Yang, Chao Xia, Fei Liao, Xiaolei Hu, Xiang Zhang. An irreversible labeling reagent for GST, CN106754853B, patented Jan. 4, 2020.
BOOK CHAPTER
1. Xiaolan Yang, Gaobo Long, Hua Zhao and Fei, Liao *. Kinetic Analyses of Enzyme Reaction Curves with New Integrated Rate Equations and Applications of Chemometrics in in Practical Applications (2012). Pp. 157-184. Kurt Varmuza (Ed.), ISBN: 978-953-51-0438-4, InTech
